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Phenotyping and global levels of <t>DNA</t> <t>methylation</t> in P. persica fruits susceptible and resistant to mealiness. (A) Experimental design for one resistant and one susceptible individual for mealiness. At harvest (E1), three fruits per tree were used for MethylC-Seq and RNA-Seq analysis, while another pool of fruits was stored in a cold chamber at 0 °C to promote mealiness. After 30 days of cold treatment (E3 stage), three fruits were used for MethylC-Seq and RNA-Seq analysis. At E4 stage, at least five fruits were ripened at room temperature and used for the measure of juice content. (B) Juice content (%) during four seasons in at least five fruits of an individual resistant and susceptible to mealiness, respectively. The dotted line represents a 30% threshold to consider fruits as mealy or normal. The same trees were evaluated in all seasons. (C) Absolute methylation levels (%) for cytosine contexts CpG, CHG and CHH (H = C, T or A) in normal and mealy fruits at E1 and E3.
Truseq Dna Methylation Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Phenotyping and global levels of <t>DNA</t> <t>methylation</t> in P. persica fruits susceptible and resistant to mealiness. (A) Experimental design for one resistant and one susceptible individual for mealiness. At harvest (E1), three fruits per tree were used for MethylC-Seq and RNA-Seq analysis, while another pool of fruits was stored in a cold chamber at 0 °C to promote mealiness. After 30 days of cold treatment (E3 stage), three fruits were used for MethylC-Seq and RNA-Seq analysis. At E4 stage, at least five fruits were ripened at room temperature and used for the measure of juice content. (B) Juice content (%) during four seasons in at least five fruits of an individual resistant and susceptible to mealiness, respectively. The dotted line represents a 30% threshold to consider fruits as mealy or normal. The same trees were evaluated in all seasons. (C) Absolute methylation levels (%) for cytosine contexts CpG, CHG and CHH (H = C, T or A) in normal and mealy fruits at E1 and E3.
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Phenotyping and global levels of <t>DNA</t> <t>methylation</t> in P. persica fruits susceptible and resistant to mealiness. (A) Experimental design for one resistant and one susceptible individual for mealiness. At harvest (E1), three fruits per tree were used for MethylC-Seq and RNA-Seq analysis, while another pool of fruits was stored in a cold chamber at 0 °C to promote mealiness. After 30 days of cold treatment (E3 stage), three fruits were used for MethylC-Seq and RNA-Seq analysis. At E4 stage, at least five fruits were ripened at room temperature and used for the measure of juice content. (B) Juice content (%) during four seasons in at least five fruits of an individual resistant and susceptible to mealiness, respectively. The dotted line represents a 30% threshold to consider fruits as mealy or normal. The same trees were evaluated in all seasons. (C) Absolute methylation levels (%) for cytosine contexts CpG, CHG and CHH (H = C, T or A) in normal and mealy fruits at E1 and E3.
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Phenotyping and global levels of <t>DNA</t> <t>methylation</t> in P. persica fruits susceptible and resistant to mealiness. (A) Experimental design for one resistant and one susceptible individual for mealiness. At harvest (E1), three fruits per tree were used for MethylC-Seq and RNA-Seq analysis, while another pool of fruits was stored in a cold chamber at 0 °C to promote mealiness. After 30 days of cold treatment (E3 stage), three fruits were used for MethylC-Seq and RNA-Seq analysis. At E4 stage, at least five fruits were ripened at room temperature and used for the measure of juice content. (B) Juice content (%) during four seasons in at least five fruits of an individual resistant and susceptible to mealiness, respectively. The dotted line represents a 30% threshold to consider fruits as mealy or normal. The same trees were evaluated in all seasons. (C) Absolute methylation levels (%) for cytosine contexts CpG, CHG and CHH (H = C, T or A) in normal and mealy fruits at E1 and E3.
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SAS institute sas proc glimmix
Phenotyping and global levels of <t>DNA</t> <t>methylation</t> in P. persica fruits susceptible and resistant to mealiness. (A) Experimental design for one resistant and one susceptible individual for mealiness. At harvest (E1), three fruits per tree were used for MethylC-Seq and RNA-Seq analysis, while another pool of fruits was stored in a cold chamber at 0 °C to promote mealiness. After 30 days of cold treatment (E3 stage), three fruits were used for MethylC-Seq and RNA-Seq analysis. At E4 stage, at least five fruits were ripened at room temperature and used for the measure of juice content. (B) Juice content (%) during four seasons in at least five fruits of an individual resistant and susceptible to mealiness, respectively. The dotted line represents a 30% threshold to consider fruits as mealy or normal. The same trees were evaluated in all seasons. (C) Absolute methylation levels (%) for cytosine contexts CpG, CHG and CHH (H = C, T or A) in normal and mealy fruits at E1 and E3.
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Image Search Results


Phenotyping and global levels of DNA methylation in P. persica fruits susceptible and resistant to mealiness. (A) Experimental design for one resistant and one susceptible individual for mealiness. At harvest (E1), three fruits per tree were used for MethylC-Seq and RNA-Seq analysis, while another pool of fruits was stored in a cold chamber at 0 °C to promote mealiness. After 30 days of cold treatment (E3 stage), three fruits were used for MethylC-Seq and RNA-Seq analysis. At E4 stage, at least five fruits were ripened at room temperature and used for the measure of juice content. (B) Juice content (%) during four seasons in at least five fruits of an individual resistant and susceptible to mealiness, respectively. The dotted line represents a 30% threshold to consider fruits as mealy or normal. The same trees were evaluated in all seasons. (C) Absolute methylation levels (%) for cytosine contexts CpG, CHG and CHH (H = C, T or A) in normal and mealy fruits at E1 and E3.

Journal: Frontiers in Plant Science

Article Title: Identification of DNA Methylation and Transcriptomic Profiles Associated With Fruit Mealiness in Prunus persica (L.) Batsch

doi: 10.3389/fpls.2021.684130

Figure Lengend Snippet: Phenotyping and global levels of DNA methylation in P. persica fruits susceptible and resistant to mealiness. (A) Experimental design for one resistant and one susceptible individual for mealiness. At harvest (E1), three fruits per tree were used for MethylC-Seq and RNA-Seq analysis, while another pool of fruits was stored in a cold chamber at 0 °C to promote mealiness. After 30 days of cold treatment (E3 stage), three fruits were used for MethylC-Seq and RNA-Seq analysis. At E4 stage, at least five fruits were ripened at room temperature and used for the measure of juice content. (B) Juice content (%) during four seasons in at least five fruits of an individual resistant and susceptible to mealiness, respectively. The dotted line represents a 30% threshold to consider fruits as mealy or normal. The same trees were evaluated in all seasons. (C) Absolute methylation levels (%) for cytosine contexts CpG, CHG and CHH (H = C, T or A) in normal and mealy fruits at E1 and E3.

Article Snippet: Libraries were generated with the TruSeq DNA Methylation kit (Illumina, San Diego, CA, United States) according to manufacturer’s instructions.

Techniques: DNA Methylation Assay, RNA Sequencing, Methylation

DNA methylation levels across the eight pseudomolecules of P. Persica. (A) Methylation levels considering all cytosine contexts in normal fruits (blue line) and mealy fruits (green line). Horizontal green bars represent the location of previously identified QTLs for mealiness, while dashed vertical lines indicates the approximate location of centromeres. (B) Methylation levels in CpG, CHG, and CHH contexts within chromosome 4, the main candidate associated with mealiness phenotype based on QTL studies.

Journal: Frontiers in Plant Science

Article Title: Identification of DNA Methylation and Transcriptomic Profiles Associated With Fruit Mealiness in Prunus persica (L.) Batsch

doi: 10.3389/fpls.2021.684130

Figure Lengend Snippet: DNA methylation levels across the eight pseudomolecules of P. Persica. (A) Methylation levels considering all cytosine contexts in normal fruits (blue line) and mealy fruits (green line). Horizontal green bars represent the location of previously identified QTLs for mealiness, while dashed vertical lines indicates the approximate location of centromeres. (B) Methylation levels in CpG, CHG, and CHH contexts within chromosome 4, the main candidate associated with mealiness phenotype based on QTL studies.

Article Snippet: Libraries were generated with the TruSeq DNA Methylation kit (Illumina, San Diego, CA, United States) according to manufacturer’s instructions.

Techniques: DNA Methylation Assay, Methylation

Average distribution of global DNA methylation throughout coding regions and number of differentially methylated regions. (A) DNA methylation level in annotated genes, including 2 kb upstream and downstream of the transcription start site (TSS) and transcription end site (TES), respectively. Each color represents a different condition. (B) DNA methylation level in annotated transposable elements (TE), including 2 kb upstream and downstream of TSS and TES. (C) Number of Differentially Methylated Regions (DMRs) close to annotated genes (up to 2 kb upstream and downstream) in normal fruits at E3 vs. E1 stages, mealy fruits at E3 vs. E1, mealy fruits vs. normal fruits at E1 and mealy fruits vs. normal fruits at E3. Blue bars indicate hypermethylated regions and red bars hypomethylated regions. DMRs where considered in all cytosine contexts and individual contexts (CpG, CHG, and CHH). Three biological replicates, a log2 enrichment difference >1 and a p -value < 0.05 were considered to identify a DMR.

Journal: Frontiers in Plant Science

Article Title: Identification of DNA Methylation and Transcriptomic Profiles Associated With Fruit Mealiness in Prunus persica (L.) Batsch

doi: 10.3389/fpls.2021.684130

Figure Lengend Snippet: Average distribution of global DNA methylation throughout coding regions and number of differentially methylated regions. (A) DNA methylation level in annotated genes, including 2 kb upstream and downstream of the transcription start site (TSS) and transcription end site (TES), respectively. Each color represents a different condition. (B) DNA methylation level in annotated transposable elements (TE), including 2 kb upstream and downstream of TSS and TES. (C) Number of Differentially Methylated Regions (DMRs) close to annotated genes (up to 2 kb upstream and downstream) in normal fruits at E3 vs. E1 stages, mealy fruits at E3 vs. E1, mealy fruits vs. normal fruits at E1 and mealy fruits vs. normal fruits at E3. Blue bars indicate hypermethylated regions and red bars hypomethylated regions. DMRs where considered in all cytosine contexts and individual contexts (CpG, CHG, and CHH). Three biological replicates, a log2 enrichment difference >1 and a p -value < 0.05 were considered to identify a DMR.

Article Snippet: Libraries were generated with the TruSeq DNA Methylation kit (Illumina, San Diego, CA, United States) according to manufacturer’s instructions.

Techniques: DNA Methylation Assay, Methylation

Differentially expressed genes between normal and mealy fruits associated with changes in DNA methylation. (A) Expression levels of DEGs overlapping with DMRs between normal and mealy fruits at E1. DEGs are grouped as hypermethylated and hypomethylated. (B) Cytosine methylation level of a hypermethylated region in mealy fruits compared to normal fruits. The DMR of approximately 2.500 bp is located within the coding region of a CYP450 82A3 (Prupe.3G066200) gene. Color key indicates methylation levels where green represents high levels and red, low levels. (C) Expression levels of DEGs overlapping with hypermethylated regions between normal and mealy fruits at E3. (D) DNA methylation level of a hypermethylated region in mealy fruits compared to normal fruits. The DMR of approximately 120 bp is located in the promoter region of an UDP-ARABINOSE 4-EPIMERASE1 (Prupe.3G187800) gene.

Journal: Frontiers in Plant Science

Article Title: Identification of DNA Methylation and Transcriptomic Profiles Associated With Fruit Mealiness in Prunus persica (L.) Batsch

doi: 10.3389/fpls.2021.684130

Figure Lengend Snippet: Differentially expressed genes between normal and mealy fruits associated with changes in DNA methylation. (A) Expression levels of DEGs overlapping with DMRs between normal and mealy fruits at E1. DEGs are grouped as hypermethylated and hypomethylated. (B) Cytosine methylation level of a hypermethylated region in mealy fruits compared to normal fruits. The DMR of approximately 2.500 bp is located within the coding region of a CYP450 82A3 (Prupe.3G066200) gene. Color key indicates methylation levels where green represents high levels and red, low levels. (C) Expression levels of DEGs overlapping with hypermethylated regions between normal and mealy fruits at E3. (D) DNA methylation level of a hypermethylated region in mealy fruits compared to normal fruits. The DMR of approximately 120 bp is located in the promoter region of an UDP-ARABINOSE 4-EPIMERASE1 (Prupe.3G187800) gene.

Article Snippet: Libraries were generated with the TruSeq DNA Methylation kit (Illumina, San Diego, CA, United States) according to manufacturer’s instructions.

Techniques: DNA Methylation Assay, Expressing, Methylation